Table I.

Primer sequences of target genes and the internal reference gene.

Genes	Primer sequences
IFN-γ	Forward: 5′-CACAGCCCTCTCCATCAACT-3′
	Reverse: 5′-GCATCTTCTCCGTCATCTCC-3′
TNF-α	Forward: 5′-AGGCACTCCCCCAAAAGAT-3′
	Reverse: 5′-CAGTAGACAGAAGAGCGTGGTG-3′
NF-κB	Forward: 5′-TGGTGGAGAACTTTGAGCCT-3′
	Reverse: 5′-GGAATTTCCAGCAGTTTGC-3′
IL-1β	Forward: 5′-TTGACAGTGATGAGAATGACCTG-3′
	Reverse: 5′-GCTCTTGTTGATGTGGTGCTGCT-3′
iNOS	Forward: 5′-AGGGAATCTTGGAGCGAGTT-3′
	Reverse: 5′-GCAGCCTCTTGTCTTTGACC-3′
β-actin	Forward: 5′-GCCGTCCTCTCTCTGTATGC-3′
	Reverse: 5′-GGGGACAGTGTGGCTGAC-3′

IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; NF-κB, nuclear factor-κB; IL-1β, interleukin-1β; iNOS, inducible nitric oxide synthase.