Figure 2.

Effect of Ni on TNF-α resistant non-small cell lung cancer cell lines. (A) The expression of EGFR protein was measured in H460, H1299, H292 and H1975 cell lines. High dose (1 mM) Ni treatment significantly inhibited the viability of (B) H292 and (C) H1975 cells. A combination of low dose Ni treatment with TNF-α significantly suppressed the viability of (D) H292 and (E) H1975 cell lines. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 vs. the Ctrl. Ni, nimotuzumab; TNF, tumor necrosis factor; EGFR, epidermal growth factor receptor; Ctrl, control.