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High glucose upregulates miR-34a expression. RMCs were incubated with normal glucose (NG, 5.5 mmol/L glucose), high mannitol (HM, 5.5 mmol/L glucose + 24.5 mmol/L mannitol), or high glucose (HG, 30 mmol/L glucose) for 24 hours. ∗p < 0.001 versus RMCs cultured with normal glucose media. mRNA expression was determined by quantitative real-time PCR. All results represent means ± SD obtained from three independent experiments in triplicate.
