Table 1.
Features of CBL and CIPK genes in cassava.
| Gene name | Locus name | Arabidopsis ortholog/AGI No. | Protein length | pI | MW (kD) | Palmitoylation sites Amino acid (location) | Myristoylation sites Amino acid (location) |
|---|---|---|---|---|---|---|---|
| MeCBL1 | cassava4.1_016071m.g | AtCBL1/At4g17615 | 213 | 4.62 | 24.56 | C (3) | G (2) |
| MeCBL2 | cassava4.1_023888m.g | AtCBL2/At5g55990 | 223 | 4.77 | 25.84 | C (4) | G (7) |
| MeCBL4 | cassava4.1_015878m.g | AtCBL4/At5g24270 | 218 | 5.20 | 25.36 | C (8), C (10) | – |
| MeCBL5 | cassava4.1_022392m.g | AtCBL5/At4g01420 | 225 | 4.60 | 25.94 | C (8), C (10) | G (7) |
| MeCBL6 | cassava4.1_014733m.g | AtCBL6/At4g16350 | 248 | 4.70 | 28.56 | – | – |
| MeCBL8 | cassava4.1_023193m.g | AtCBL8/At1g64480 | 214 | 4.78 | 24.51 | C (7) | G (6) |
| MeCBL9 | cassava4.1_016083m.g | AtCBL9/At5g47100 | 213 | 4.76 | 24.50 | C (3) | G (2) |
| MeCBL10 | cassava4.1_014701m.g | AtCBL10/At4g33000 | 249 | 4.70 | 28.74 | C (20) | – |
| MeCIPK1 | cassava4.1_008050m.g | AtCIPK1/At3g17510 | 431 | 7.15 | 48.47 | – | G (4), G (9) |
| MeCIPK2 | cassava4.1_007534m.g | AtCIPK2/At5g07070 | 449 | 8.37 | 51.35 | – | G (5) |
| MeCIPK3 | cassava4.1_010345m.g | AtCIPK3/At2g26980 | 362 | 5.85 | 40.94 | – | – |
| MeCIPK4 | cassava4.1_008191m.g | AtCIPK4/At4g14580 | 427 | 9.12 | 47.53 | C (218) | – |
| MeCIPK5 | cassava4.1_029811m.g | AtCIPK5/At5g10930 | 448 | 8.69 | 50.74 | – | – |
| MeCIPK6 | cassava4.1_031025m.g | AtCIPK6/At4g30960 | 438 | 9.19 | 49.43 | C (236) | G (2), G (8) |
| MeCIPK7 | cassava4.1_008412m.g | AtCIPK7/At3g23000 | 420 | 9.07 | 47.08 | – | – |
| MeCIPK8 | cassava4.1_007600m.g | AtCIPK8/At4g24400 | 446 | 7.63 | 50.53 | – | G (7) |
| MeCIPK9 | cassava4.1_007907m.g | AtCIPK9/At1g01140 | 437 | 8.94 | 49.29 | – | – |
| MeCIPK10 | cassava4.1_007266m.g | AtCIPK10/At5g58380 | 459 | 8.30 | 51.94 | C (231) | – |
| MeCIPK11 | cassava4.1_034294m.g | AtCIPK11/At2g30360 | 433 | 9.10 | 49.13 | C (179) | – |
| MeCIPK12 | cassava4.1_006117m.g | AtCIPK12/At4g18700 | 499 | 6.63 | 56.05 | – | – |
| MeCIPK13 | cassava4.1_024110m.g | AtCIPK13/At2g34180 | 450 | 9.12 | 50.94 | – | G (5) |
| MeCIPK14 | cassava4.1_008116m.g | AtCIPK14/At5g01820 | 429 | 7.08 | 48.24 | – | – |
| MeCIPK15 | cassava4.1_006767m.g | AtCIPK15/At5g01810 | 476 | 8.92 | 53.95 | – | G (5) |
| MeCIPK16 | cassava4.1_028375m.g | AtCIPK16/At2g25090 | 453 | 8.66 | 50.87 | – | G (5) |
| MeCIPK17 | cassava4.1_007161m.g | AtCIPK17/At1g48260 | 463 | 8.79 | 51.89 | – | G (5), G (10) |
| MeCIPK18 | cassava4.1_007849m.g | AtCIPK18/At1g29230 | 438 | 9.05 | 49.11 | C (236) | G (8) |
| MeCIPK19 | cassava4.1_006740m.g | AtCIPK19/At5g45810 | 477 | 8.08 | 53.63 | – | – |
| MeCIPK20 | cassava4.1_025576m.g | AtCIPK20/At5g45820 | 454 | 9.20 | 51.32 | – | G (7) |
| MeCIPK21 | cassava4.1_006970m.g | AtCIPK21/At5g57630 | 469 | 6.08 | 52.97 | C (379), C (383) | G (2) |
| MeCIPK22 | cassava4.1_030445m.g | AtCIPK22/At2g38490 | 398 | 9.02 | 44.72 | – | – |
| MeCIPK23 | cassava4.1_007136m.g | AtCIPK23/At1g30270 | 463 | 8.95 | 51.77 | C (302) | G (5) |
| MeCIPK24 | cassava4.1_007604m.g | AtCIPK24/At5g35410 | 447 | 8.29 | 50.17 | C (441) | – |
| MeCIPK25 | cassava4.1_007324m.g | AtCIPK25/At5g25110 | 457 | 8.79 | 51.76 | – | – |
| MeCIPK26 | cassava4.1_007827m.g | AtCIPK26/At5g21326 | 439 | 6.88 | 50.26 | – | – |
The nomenclature of MeCBL and MeCIPK followed that of Arabidopsis CBL and CIPK proteins through a comparison using MEGA 5.0 software. The protein length, isoelectric point (pI) and molecular weight (MW) of the encoded proteins were predicted by ExPASy tool (http://web.expasy.org/protparam/). The palmitoylation sites and myristoylation sites were predicted by CSS-Palm 3.0 (http://csspalm.biocuckoo.org/), G represent the glycine residue, and C represent the cysteine residue.