Table 2.
Interaction of MeCBLs and MeCIPKs in yeast two-hybrid assay.
| MeCBL1 | MeCBL2 | MeCBL4 | MeCBL5 | MeCBL6 | MeCBL8 | MeCBL9 | MeCBL10 | |
|---|---|---|---|---|---|---|---|---|
| MeCIPK1 | − | − | − | − | + | + | + | + |
| MeCIPK2 | + | + | + | − | + | − | − | − |
| MeCIPK3 | − | − | − | + | + | − | − | − |
| MeCIPK4 | − | − | − | + | + | − | − | − |
| MeCIPK5 | − | − | − | − | + | − | − | + |
| MeCIPK6 | − | − | − | − | − | − | − | − |
| MeCIPK7 | − | + | + | − | + | − | − | − |
| MeCIPK8 | + | − | − | + | + | − | + | + |
| MeCIPK9 | − | − | − | − | + | − | + | − |
| MeCIPK10 | + | + | + | + | + | + | + | − |
| MeCIPK11 | − | − | − | − | − | − | − | − |
| MeCIPK12 | − | + | − | − | − | − | − | − |
| MeCIPK13 | − | + | − | − | + | − | − | − |
| MeCIPK14 | + | − | + | + | + | + | + | − |
| MeCIPK15 | + | + | − | − | + | + | − | − |
| MeCIPK16 | + | + | + | − | + | + | + | − |
| MeCIPK17 | + | − | − | + | − | − | − | − |
| MeCIPK18 | + | − | + | − | + | + | + | + |
| MeCIPK19 | + | − | + | + | + | + | + | + |
| MeCIPK20 | − | − | − | − | + | − | − | − |
| MeCIPK22 | − | − | + | − | + | − | − | + |
| MeCIPK23 | + | + | − | − | + | − | + | + |
| MeCIPK24 | − | + | − | − | + | − | − | + |
| MeCIPK25 | − | − | − | − | − | − | + | − |
| MeCIPK26 | + | − | − | − | + | + | + | − |
The interaction analyses of MeCBL and MeCIPK proteins were performed using yeast two-hybrid system. Eight MeCBLs and 25 MeCIPKs was cloned and inserted into the pGADT7 and pGBKT7 plasmid, respectively. The plasmids were transformed into the yeast strain Y2HGold through lithium acetate method, and the interacting relationships were detected by the yeast growth on non-selective medium (DDO: SD-T-L) and selective medium (QDO+X+AbA: SD-T-L-H-A+40 μg/mL X-α-Gal + 125 ng/mL Aureobasidin A). + represents growth (interaction), − represents no growth (no interaction).