GluCl activation increases thermal and mechanical pain thresholds. (A) Von Frey test of mechanical sensitivity (β only, n = 14 animals and αβ, n = 13); (B) Hargreaves test of thermal sensitivity (β only, n = 14 animals and αβ, n = 13); (C) Pinprick assay of noxious mechanosensation (β only, n = 11 animals and αβ, n = 10); (D) Rotarod test of motor performance (β only, n = 14 animals and αβ, n = 13); and (E) beam walk test of proprioception (β only, n = 8 animals and αβ, n = 9) of GluCl AAV animals before and 24 h following 5 mg/kg ivermectin (IVM). Student’s paired t-test. (F) Time course of thermal sensitivity following a single dose of systemic ivermectin. Repeated measures ANOVA followed by post hoc Bonferroni test comparing β only (n = 12) and αβ (n = 10.) (E) Ivermectin-induced change in thermal sensitivity of two separate doses of ivermectin given 24 h prior to testing, performed 3 weeks apart. Student’s paired t-test, n = 6 animals. All averaged data represent mean ± SEM. *P < 0.05, **P < 0.01. BS = baseline.