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Chemoattraction protocol
| Somal compartment | Neurite compartment | |
|---|---|---|
| Day 0 | 50 ng/ml NGF, 10 ng/ml GDNF in 200 µl | 50 ng/ml NGF, 10 ng/ml GDNF in 200 µl |
| Day 1 | 5 ng/ml NGF, 1 ng/ml GDNF in 160 µl | 50 ng/ml NGF, 10 ng/ml GDNF in 200 µl |
| Day 2 | 0 ng/ml NGF, 0 ng/ml GDNF in 160 µl | 50 ng/ml NGF, 10 ng/ml GDNF in 200 µl |
| Days 3 and 5 | 0 ng/ml NGF, 0 ng/ml GDNF in 160 µl | 50 ng/ml NGF, 10 ng/ml GDNF in 200 µl |
