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. 2018 Mar 7;8:4123. doi: 10.1038/s41598-018-22401-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of MSCs isolated from pig adipose tissue. (A) Representative flow cytometry of MSCs isolated and propagated from adipose tissue for up to 5 passages. Counts with specific immunoreactivity are shown in pale blue. Counts stained with isotype control antibodies are shown in red. (B) Live cells were >70% immunoreactive for CD44, CD73, CD90, CD105, and CD146 and <5% immunoreactive for CD14, CD45, CD106, CD271, and SLA-II DR (N = 3 different pigs). (C) In each culture, many cells could form colonies from single cells (N = 8). (D) Representative brightfield image of cells attached to a tissue culture flask. (E,F) Immunocytochemistry showed that the majority of MSCs expressed the immature cell marker, nestin (red, E) and the actin isoform, alpha smooth muscle actin (alpha SMA, green, F). (G–I) Upon directed differentiation, subpopulations of cells contained mucins (G), lipid droplets (H) or produced calcium (I). Scale bar; D,F = 50 μm, E, H, I = 40 μm, G = 100 μm.