Skip to main content
. 2018 Mar 7;9(3):377. doi: 10.1038/s41419-018-0405-4

Fig. 4. FR054 induces in MDA-MB-231 cells a decrease in N-/O-GlcNAc protein levels and a reduction of their ability to adhere and migrate.

Fig. 4

FACS analysis of membrane N-glycans in live cells, treated with FR054 or grown in 1 mM glucose, stained with fluorochrome-conjugated ConA (a) and PHA-L (b). c Confocal microscopy of PHA-L staining in MDA-MB-231 cells treated with 250 μM FR054 for 24 h (40× magnification, 20 μm scale) and relative fluorescence intensity quantification. d Protein O-GlcNAc detection in total cell extract from MDA-MB-231 cells treated with 1 mM FR054 for 24 and 48 h and relative band intensity quantification (right histogram). e MDA-MB-231 cells appearance upon 48 h treatment with FR054 (4× magnification, 50 μM scale). f Confocal microscopy analysis of β1 active integrin membrane localization in MDA-MB-231 cells treated with 250 μM FR054 for 24 h (40× magnification, 20 μm scale) and relative fluorescence intensity quantification. g β1 integrin expression in MDA-MB-231 cells upon treatment with 250 μM FR054 for 24 h. MDA-MB-231 cell adhesion (h) and migration (i), upon 24 h treatment with 250 μM FR054. All data represent the average ± s.d.; *p < 0.05, **p < 0.01 (Student’s t-test; comparison with FR054-not-treated sample); N = 3