Fig. 2. BKM120 kills cells via the intrinsic apoptosis pathway.

a Annexin-V-FITC/PI assay in MINO and JEKO-1 cells in response to 2 µM BKM120 for 48 h and concomitant blockage of caspases with 10 µM QV-D-OPh. Percentages refer to cells positive for both, PI and Annexin-V-FITC staining (upper right quadrant) and cells positive only for Annexin-V-FITC staining (lower right quadrant), respectively. Results are representative for three independent experiments. b Measurement of the protein levels of Bax and Bak and cleavage/activation of caspase-9 in MINO cells in response to 1 µM and 1.5 µM of BKM120 incubated for 24 h. c Relative mRNA expression levels of Bcl-2 family members after incubation of JEKO-1 cells with 1.5 µM of BKM120 for the indicated time points [h]. Mean ± SD of three independent experiments