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. 2018 Mar 7;9(3):384. doi: 10.1038/s41419-018-0413-4

Fig. 5. Concomitant Bax, Bax, and p53 deficiency determines polyploidy.

Fig. 5

a Basal protein expression levels of apoptotic regulators in a panel of NHL cell lines. b Annexin-V-FITC/PI assay of HCT116 WT or HCT116 Bax−/−/Bak−/− cells incubated with increasing concentrations of BKM120 for 72 h (upper panel). Annexin-V-FITC/PI assay of HCT116 WT or HCT116 Bax−/−/Bak−/− cells co-transfected with ctrl or p53 siRNA and incubated with 2.5 µM BKM120 for 72 h (lower panel). Data show mean ± SD of three independent experiments. c Polyploidy assay of HCT116 WT or HCT116 Bax−/−/Bak−/− cells co-transfected with ctrl or p53 siRNA and incubated with increasing concentrations of BKM120 for 72 h. Data show mean ± SD of three independent experiments. *p < 0.05; **p < 0.005