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. 2018 Mar 7;9(3):384. doi: 10.1038/s41419-018-0413-4

Fig. 7. BKM120-induced mitotic arrest and polyploidy requires MEK1/2.

Fig. 7

a Expression of phosphorylated MEK1/2 in MINO and DG-75 after treatment with increasing concentrations of BKM120 for 3 h. b Expression of Cyclin B1 and phosphorylated CDK1 in MINO and DG-75 cells pretreated with 50 µM U0126 for 4 h and subsequently incubated with 1 µM BKM120 for 24 h. c Measurement of cell cycle distribution and polyploidy of MINO and DG-75 cells pretreated with 50 µM U0126 for 4 h and subsequently incubated with 1 µM BKM120 for 72 h. Quantification with ModFit LT. Data shown are representative for two independent experiments. d WB analysis of MEK1/2 phosphorylation and Cyclin B1 expression in MINO and DG-75 cells upon treatment with 1.5 µM BKM120, 20 µM LY294002, 5 µM BEZ235, or 100 µM Wortmannin