Fig. 3. Characteristics of liver phenotype in WT and Foxo3^−/− after 10 days of ethanol feeding.

WT and Foxo3^−/− mice were either chow-fed (Naïve), pair-fed or ethanol-fed with the Lieber-DeCarli diet for 10 days and liver injury was evaluated by serum ALT level a, liver body ratio and fat accumulation b Hepatic mRNA levels of TNF-α, IL-6, MCP-1, IL-4 c and the classical macrophage marker iNOS and alternative macrophage markers Arg1 and Fizz-1 d were measured by real time RT-PCR. n = 3–4. e–f LysM Foxo3 and control mice were pair-fed or fed with ethanol diet for 10 days, hepatic mRNA levels of TNF-α, IL-6, IL-1β, IL-4, iNOS and alternative macrophage markers Arg1 and Ym-1 were measured by real time RT-PCR e Serum level of TNF-α, IL-6, and MCP-1 were analyzed by ELISA f n = 3. All data were statistically analyzed with two-tailed Student’s t test, and presented as mean ± SEM. *P < 0.05, **P < 0.01