Fig. 5. Foxo3^−/− mice displayed higher macrophage-related liver inflammation after 10 days ethanol feeding.

a–c Mice were fed with control or ethanol diet as for Fig. 3. a Hepatic mRNA level of F4/80, CD11b, CD123, and Ly6C was measured by real time RT-PCR. n = 3. b Co-staining of Ly6C and CD11b in liver sections. n = 3. The right panels are higher magnifications of the merged images. c IHC staining for Ly6C and Co-staining of Ly6C and CD11b in liver sections from LysM Foxo3 and control mice were pair-fed or fed with ethanol diet for 10 days. n = 3. d–e Mice were fed with control or ethanol for 10 days and CD11b⁺ cells were isolated from the liver, mRNA levels of TNF-α, IL-6, CCl-2, iNOS and Arg1 were measured by real time RT-PCR. n = 3. b CD11b⁺ cells as in A were treated with LPS (10 ng/mL) for 6 h. TNF-α, IL-6 and MCP-1 levels were measured by ELISA. n = 3. All data were statistically analyzed with two-tailed Student’s t test, and presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001