Fig. 6. Restoring macrophage apoptosis ameliorates pro-inflammatory phenotype in Foxo3−/− mice after ethanol feeding.
a Schematic representation of the time course protocol of the experiment. b TUNEL assays from liver sections after injection at indicated times. n = 3. c IHC staining for F4/80 in liver sections at varies of time as indicated. n = 3. d Hepatic mRNA levels of Ly6C, TNF-α, IL-1β, IL-6, IL-4, iNOS, Arg1, and Ym-1 at 9 dpi from mice that had received injections of either NaCl or GdCl3. n = 3. e Serum levels of TNF-α, IL-6, and MCP-1 at 9 dpi from Foxo3−/− mice that received injections of either NaCl or GdCl3. n = 3. All data were statistically analyzed with two-tailed Student’s t test, and presented as mean ± SEM. *P < 0.05, **P < 0.01