Fig. 3. Nuclear-resident Akt activation by Na⁺-free exposure in nuclear preparations from neuronal cells.

a Representative Western blot and quantification of phosphorylated Akt in isolated nuclei from NGF-differentiated PC12 cells (3 days) after perfusion with Na⁺-free or bradykinin (BK). b Representative Western blot and quantification of phosphorylated Akt in isolated nuclei from NGF-differentiated PC12 cells previously treated with the PI3’K inhibitor LY294002 c Representative Western blot and quantification of the effect of Na⁺-free (5 min) in isolated nuclei obtained from NGF-differentiated PC12 cells (3 days) previously treated with siControl or siNCX1. For a, b, c: all the experiments were repeated at least three times on different nuclear preparations. *p < 0.05 vs. untreated control or siControl, both not exposed to Na⁺-free. **p < 0.05 vs. control or siControl + 5′ Na⁺-free