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. 2018 Apr 30;4:12. doi: 10.1038/s41420-017-0018-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Scheme of XIP-NLS structure. b Immunolocalization of XIP-NLS (green, 400 ng/ml) detected 48 h after transfection. Hoechst dye (blue) was used to mark nuclei. Under these conditions, EYFP-positive nuclei were at least 25 ± 2% of total Hoechst-positive nuclei. c Western blot of EYFP, CREB, pCREB, and SOD1 in cytosolic and nuclear fractions obtained from NGF-differentiated PC12 cells transfected with XIP-NLS (400 ng/ml). Fractions were prepared 48 h after transfection