Table 1.
Primer sequences used for RT-qPCR
| Molecular names | Primer sequences | Enzymes |
|---|---|---|
| miR122-RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCAAACAC | |
| miR122-F | GGGGTGGAGTGTGACAATG | |
| miR122-R | CAGTGCGTGTCGTGGAGT | |
| U6-RT | AACGCTTCACGAATTTGCGT | |
| U6-F | CTCGCTTCGGCAGCACA | |
| U6-R | AACGCTTCACGAATTTGCGT | |
| miR192-RT | GCGTATCACCGTGTTTACTGTGGCTGTCA | |
| miR192-F | GCCCGTATCTGACCTATGAATTGA | |
| miR192-R | AGCGTATCACCGTGTTTACTGT | |
| miR204-RT | CACATTAGCGCGTATTCAGACAGGCATAGG | |
| miR204-F | CCACTTTGATTCCCTTTGTCATCC | |
| miR204-R | CCACATTAGCGCGTATTCAGAC | |
| circ_0001498-F | CATTTCCTTCCTTGGAATCTTG | |
| circ_0001498-R | CTGGGCATTCCACACAACTA | |
| circ_0008026-F | TGGCACACCTGGATATCTTTC | |
| circ_0008026-R | GCAAATTCTCAGGCTTCAGG | |
| circ_406626-F | CCTCAATATTTTTACTGGCCACA | |
| circ_406626-R | ATCTTCATTCCTCAATATTTTTACTGG | |
| MAP3K2-F | GCAAAGTTCTGTGCCAGTGA | |
| MAP3K2-R | ACTGTTTGCCCATGTTCCTC | |
| GLS-F | GCTGTGCTCCATTGAAGTGA | |
| GLS-R | AAAGCAAACTGCCCTGAGAA | |
| TGFBRAP1-F | CGTTCACTGCCACCAAACAG | |
| TGFBRAP1-R | CTCGTTCAGTGCAAACGTGG | |
| CPEB1-F | ATCCTTGCTTCAGGCTTGCT | |
| CPEB1-R | CATGCAGAGCACCGACAAAC | |
| FRS2-F | CATTGGAGGCGAGGGTTTCT | |
| FRS2-R | AGCCAGTGTCCCATTCTGTG | |
| FN1-F | AGCCTGGGAGCTCTATTCCA | |
| FN1-R | TTGGCGCATCTCTTTCCTGT | |
| HPCAL1-F | ACGGCAAACTGTCCTTGGAA | |
| HPCAL1-R | GCAAAGCTTAAACGGCACGA | |
| miR122-psu-F | GGAAGATCTTGTTCAGCTCTTCCCATTGCT | BglII |
| miR122-psu-R | CCCAAGCTTTGAGTGCAAAAGAGCCAGACT | HindIII |
| miR192-psu-F | GGAAGATCTTCACGGGGAATGAGACAGAG | BglII |
| miR192-psu-R | CCCAAGCTTCCAGTGGGGCTGCTGTTAT | HindIII |
| miR204-psu-F | GGAAGATCTTGAGAGTATATTTCACTCCTTCCT | BglII |
| miR204-psu-R | CCCAAGCTTACTTCCTGATCGCGTACC | HindIII |
Note: Underline represents the restriction enzyme cutting site.
Abbreviations: CPEB1, cytoplasmic polyadenylation element binding protein 1; F, forward; FN1, fibronectin 1; FRS2, fibroblast growth factor receptor substrate 2; GLS, glutaminase; HPCAL1, hippocalcin like 1; MAP3K2, mitogen-activated protein kinase 2; psu, precursor primer for amplification of miRNA; R, reverse; RT-qPCR, quantitative real-time PCR; TGFBRAP1, transforming growth factor β receptor-associated protein 1.