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. 2018 Mar 2;11:1157–1171. doi: 10.2147/OTT.S137146

Figure 4.

Figure 4

Overexpression of SIRT1 inhibits OPN-induced cell proliferation.

Notes: (A and B) A549 and NCI-H358 cells infected with LV-SIRT1 or LV-NC were treated with OPN (0, 100, 200, 400 ng/mL) for 24 hours; cell proliferation was examined by CCK-8 assay. The group treated with CB + LV-NC served as control. The absorbance in control group is presented as 1. (C and D) A549 and NCI-H358 cells infected with LV-SIRT1 or LV-NC were treated with OPN (400 ng/mL). Cell viability was examined by CCK-8 assay at 0, 12, 24, 48 hours after OPN treatment. The absorbance at 0 hour serving as control is presented as 1. Results presented are mean ± SD analyzed with Student’s t-test from at least three independent experiments. *P<0.05, **P<0.01, ***P<0.001 OPN + LV-NC versus CB + LV-NC. ##P<0.01, ###P<0.001 OPN + LV-SIRT1 versus OPN + LV-NC.

Abbreviations: SIRT1, Sirtuin 1; OPN, osteopontin; LV-SIRT1, lentiviral vectors overexpressing SIRT1; NC, negative control; CCK-8, Cell Counting Kit-8; CB, control buffer; SD, standard deviation.