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. 2018 Feb 20;4:29. doi: 10.1038/s41420-018-0035-8

Fig. 6. Knockdown of G9a sensitizes Caki cells to TRAIL-mediated apoptosis.

Fig. 6

a Caki cells were transiently transfected control siRNA (Cont siRNA) or G9a siRNA. Twenty-four hours after transfection, cells were treated with 50 ng/ml TRAIL for 24 h. Apoptosis was analyzed as a sub-G1 population by flow cytometry (upper panel). The protein levels of PARP, G9a, and actin were determined by western blotting. (lower panel). b Caki cells were transiently transfected control siRNA (Cont siRNA) or G9a siRNA or treated with 10 μM BIX for 24 h. The protein levels of G9a, XIAP, survivin, DR5, and actin were determined by western blotting. c Caki cells were transiently transfected control siRNA (Cont siRNA) or G9a siRNA. Twenty-four hours after transfection, cells were treated 50 ng/ml TRAIL in the presence or absence of 10 μM BIX for 24 h. Apoptosis was analyzed as a sub-G1 population by flow cytometry. The protein levels of PARP, G9a, and actin were determined by western blotting. The level of actin was used as a loading control. The values in a represent the mean ± SD from three independent samples; *p < 0.01 compared with TRAIL-treated control siRNA. **p < 0.01 compared with control-control siRNA. #p < 0.01 compared with BIX plus TRAIL-treated control siRNA