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. 2018 Mar 7;9(3):379. doi: 10.1038/s41419-018-0420-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Cytotoxicity of 327 and Scr-327 toward various human cancer cells following treatment with peptide for 12 h, as measured by CCK-8 assays (n = 3). b Colony formation ability of cancer cells following treatment with peptide 327 and Scr-327 (100 μM). c Apoptosis of cancer cells treated with peptide 327 and Scr-327 (100 μM) for 12 h was assessed by using flow cytometry. Data obtained from the Annexin V-APC/PI double staining and flow cytometry assay shows apoptosis levels. d Migrated activity of cancer cells in the absence or presence of peptide 327 (100 μM) in the transwell assay, and the quantification of migrated cells through the membrane is shown as a proportion of their vector control (n = 3)