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. 2018 Mar 7;8:4158. doi: 10.1038/s41598-018-22554-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Human hippocampus shows no difference in the number of neurons, expression of apoptotic marker or NPY-interneuron number in interface incubation. (A) Neuronal nuclear marker, NeuN in green (scalebar 500 μm), outlining cell layers magnified to the right for CA1 and dentate gyrus (scalebar 100 μm). (B) No differences were found in number of NeuN-positive neurons in the dentate granular layer over the time studied (green, scalebar 100 μm, Patient 16), (C) nor in the number of neurons positive for the apoptotic marker Cas3 (red). (D) Example of NPY-expressing interneurons positive for NeuN (green) and NPY (red) found in the hilar region of dentate gyrus from 48 h time-point. Scalebar 20 μm.