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. 2018 Jan 29;4:2. doi: 10.1038/s41420-017-0005-6

Fig. 3. AIF disease-causing mutation has limited effect on C. elegans lifespan.

Fig. 3

a Multiple amino acid alignment of WAH-1/AIF region consisting of Pro277 to Glu314. The human Arg201 (red arrow) is conserved across species. b–c Lifespan assay of wild-type (black line) and nematode expressing WAH-1 (R309 del) variant (red and orange lines). Although BAN271 and BAN272 show a reduced lifespan of a few days, outcrossed animals have the same lifespan as wild-type ones. Solid black line, wild-type N2; red solid line, BAN271; red dashed line, BAN272; orange dotted line, BAN278; orange dashed line, BAN279; orange solid line, BAN280. d Cumulative lifespan experiment plot of wild type and WAH-1 mutant animals (**p < 0.01, ****p < 0.0001; NS, nonsignificant). e Lifespan table relative to experiments in Fig. 3b-c. f OCR in wild type and WAH-1 (R309 del) mutant nematodes. g Statistical analysis of basal respiration of wild type and WAH-1 (R309 del) mutant nematodes. NaN3 was used to inhibit mitochondrial respiration (**p < 0.01)