Fig. 4. Ectopic expression of SLC2A5 exacerbates the phenotypes of LUAD cells.

a Measurement of SLC2A5 overexpression in A549 and H1299 cells transduced with lentiviruses of expressing EGFP (control) and two different SLC2A5 transcript variants, transcript variant 2 (TV2) and 5 (TV5) by real-time PCR. b Proliferation of A549 and H1299 cells expressing control or SLC2A5 transcript variants in medium containing 25 mM glucose or fructose. c Overexpression of SLC2A5 increased mobility of LUAD cells. d Ectopic expression of SLC2A5 enhanced cell invasion of LUAD cells in medium containing 25 mM fructose. e Colony formation of LUAD cells expressing control or SLC2A5 transcript variants in soft agar fed with complete medium containing 25 mM fructose. N = 3; *p < 0.05; **p < 0.01