Fig. 4.

Acetobacter decreases TAG stores and shortens lifespan. a Experimental strategy for G418 treatment. b 16S rRNA-sequencing analysis of G418 (25 mg/L)-treated w^iso31 larval guts. Each column represents a biological replicate and colours indicate OTUs. c Quantitative PCR using species-specific primers of bacteria in w^iso31 male flies raised on tBH (0.5 mM, red), paraquat (PQ; 1 mM, blue) or G418 (25 mg/L, green). Mean ± SEM (n = 5 except PQ (n = 3)). d, e Whole-body TAG (d) or lifespan (e) of w^iso31 male flies raised on tBH (0.5 mM, red) or G418 (25 mg/L, green). Mean ± SEM (n = 6). *p < 0.05, **p < 0.01. Statistics for survival curves are shown in Supplementary Table 1