Fig. 5.

Transmission of the tBH microbiome, TAG storage and longevity to the next generation. a Outline of experimental strategy. Male and female tBH-experienced flies (F0) were mated for 0–5 days after eclosion, transferred to a new bottle and eggs collected for 4 h to establish the next generation (F1). b qPCR analysis of bacteria from F1 male flies using species-specific primers. Mean ± SEM (n = 5). c Whole-body TAG (mg/mg protein) in F1 male flies. Mean ± SEM (n = 4 for control and 6 for tBH). d Lifespan of F1 male flies. ***p < 0.001. Statistics for survival curves are shown in Supplementary Table 1