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. 2018 Feb 14;4:25. doi: 10.1038/s41420-018-0028-7

Fig. 3. Change of GRK2 intracellular levels affects mitochondrial respiration after IR exposure.

Fig. 3

a–c GRK2 silencing impairs mitochondrial respiration and recovering after IR exposure in HEK293. Representative graphs of n = 2 independent experiments of HEK293 cells treated with scramble-siRNA and siRNA-GRK2 in basal conditions, at 3 and 8 h post IR exposure. HEK293 cells treated with siRNA-GRK2 have a compromised OCR already in basal condition compared to control cells (a). IR exposure blunts mitochondrial respiration at 3 h in both conditions (b) but with no recovery at 8 h post-IR in cells with GRK2 silencing (c). d–f. GRK2 preserves mitochondrial respiration and facilitates recovering after IR exposure in HEK293. Representative graphs of n = 2 independent experiments of HEK293 cells treated with pcDNA3.1 and pcDNA3.1-GRK2 at basal, 3 h and 8 h post IR. GRK2 overexpression enhances mitochondrial respiration at basal condition (d) and preserves mitochondrial respiration at 3 h post-IR (e). Full recovery of mitochondrial respiration at 8 h post-IR in both conditions is enhanced by GRK2 overexpression as compared to control cells (f)