Fig. 1. Evaluation of the radiomitigative effects of various FGF family proteins on irradiated IEC-6 cells.

In all experiments, FGFs were added 30 min after irradiation. Asterisks indicate statistical significance between FGF2 and vehicle. a Clonogenic survival curve for irradiated IEC-6 cells. IEC-6 cells were treated with vehicle (PBS, Gibco), FGF1, FGF2, or FGF19 (each 10 ng/ml) for 24 h after the indicated doses of irradiation. n = 3 in each point. b Cell viability measured by CCK-8 assay 48 h after 2 Gy and 4 Gy irradiation, which were normalized to vehicle-treated group. n = 3 for each group. c Analyses of signaling pathways by Western blot with cell extracts from IEC-6 cells irradiated (4 Gy) and treated with FGFs for 4 h. Data are representative results from at least two independent experiments. Values are mean ± SEM; *p < 0.05, **p < 0.01