Table 6.

Primers used for sequencing the full-length genomes of PRRSV isolates.

Fragment	Primer	Sequence (5′-3′)	Location
1	PRRSV-F1	atgacgtataggtgttggct	1–20
	PRRSV-R1	atttaccatccggttggcgat	1524–1544
2	PRRSV-F2	acaagtggtatggtgctggga	1327–1347
	PRRSV-R2	gaagcacaacatcccaatcaaagg	2194–2217
3	PRRSV-F3	aaaattgaccagtacctccg	2103–2122
	PRRSV-R3	ggcggtgtctcgagaatcatctt	3483–3505
4	PRRSV-F4	tgatgcgtgaggcatgtgatg	3328–3348
	PRRSV-R4	ggaaagaaaggagctcggaatggaa	4765–4789
5	PRRSV-F5	aaacccatcgcgtatgccc	4602–4620
	PRRSV-R5	gtctttaattatgtggctgcc	6180–6200
6	PRRSV-F6	cattgttacgcgcccttcagg	6071–6091
	PRRSV-R6	gcggccacagcgggtcaagc	7740–7759
7	PRRSV-F7	tcggtatgatgaacgttgacg	7621–7641
	PRRSV-R7	ggtagtttggcatggagggag	9268–9288
8	PRRSV-F8	ttacttcaaaagcggtcaccc	9146–9166
	PRRSV-R8	ggtgaggacttgcccatatt	10582–10601
9	PRRSV-F9	ctgaagaccatctggagatt	10464–10483
	PRRSV-R9	gggcatctcaccatggtatg	11834–11853
10	PRRSV-F10	atttggacccctgcatggccct	11714–11735
	PRRSV-R10	gacaaaaacgccaaccaagc	13051–13070
11	PRRSV-F11	gctcatggtgaattatacggtgt	12864–12886
	PRRSV-R11	cgaaaaattgcatgtcctggcgc	14201–14225
12	PRRSV-F12	tttcctgtgttgactcatattg	14026–14047
	PRRSV-R12	ttttttttttaatttcggccgcatggttct	polyA