Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 7;9(3):381. doi: 10.1038/s41419-018-0423-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a The diagram shows that four differentially expressed proteins, which downregulated in the sdhA mutant (EDL933:ΔsdhA) and upregulated in the sdhA complement strain (EDL933:ΔsdhA-pWF134), were identified by our proteomic analysis. b The mRNA levels of tnaA and dnaJ in EHEC wild-type strain (EDL933), EDL933:ΔsdhA mutant strain (EDL933:ΔsdhA) the sdhA gene complementation strain (EDL933:ΔsdhA-pWF134) and 2.5 mM fumarate-treated sdhA (EDL933:ΔsdhA + Fumarate) were examined. Relative transcriptional expression was normalized to the expression of rpoA. The asterisk denotes statistically significant (P < 0.0001) examined by the t-test, and error bars indicate the SEM of three independent experiments. c The survival of N2 worms fed with the wild-type strain (EDL933) and the isogenic deletion strains of tnaA (EDL933:ΔtnaA) and sdhA (EDL933:ΔsdhA) were examined. Deletions of tnaA (median N2 lifespan = 8.0 ± 0.1 days, P < 0.0001) and sdhA (median N2 lifespan = 12.4 ± 1.8 days, P < 0.0001) all conferred the attenuated toxic phenotype compared to the wild-type EDL933 (median N2 lifespan = 6.4 ± 0.9 days). d The survival of N2 worms fed with the wild-type strain (EDL933) and the isogenic deletion strains of tnaA (EDL933:ΔtnaA), sdhA (EDL933:ΔsdhA), and the sdhA and tnaA double mutant (EDL933:ΔsdhAΔtnaA) were examined. The virulence of sdhA and tnaA double mutant (EDL933:ΔsdhAΔtnaA, median N2 lifespan = 13.5 ± 0.7 days) was similar to the EDL933:ΔsdhA (median N2 lifespan = 13.5 ± 2.1 days, P = 0.474). The asterisk denotes statistically significant (P < 0.0001) examined by the log-rank test, and “ns” represents no significant difference statistically