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. 2018 Feb 14;4:24. doi: 10.1038/s41420-018-0032-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The chemical structure of compound #2714. b The CCK-8 assay was used to measure the inhibition effects of #2714 on Lewis lung carcinoma (LL/2) cells after treatment for different times and concentrations. c The EdU–DNA incorporation assay was carried out to evaluate the effect of #2714 on LL/2 cell proliferation. The fluorescence microscopic appearance of S-phase LL/2 cells were stained with Hoechst 333258 (blue) and EdU (red) after treatment with different concentrations of #2714 for 48 h. (Mean ± SD, n = 3; 100×; *p < 0.05; ***p < 0.001)