Fig. 4. Effects of compound #2714 on the expression of key proteins involved in the G2/M phase transition and on apoptosis- and proliferation-associated proteins, analyzed via western blotting.

Lewis lung carcinoma (LL/2) cells were exposed to compound #2714 for 24 h at designated gradient concentrations ranging from 2.5 to 10 μM in vitro. The expression levels of the phosphorylated forms of Cdc25C, Akt, and p44/42 MAPK in LL/2 cells were analyzed after treatment. The expression levels of p53 and cleaved caspase-3 were also assessed via western blotting. β-Actin was used as a loading control. Data are representative of three independent and parallel experiments