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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Neuroscience. 2018 Jan 31;374:155–171. doi: 10.1016/j.neuroscience.2018.01.046

Figure 9.

Figure 9

TUBB3 mutations inhibit netrin-1-induced CA attraction. A. Schematic diagram showing in ovo electroporation and an open-book CA turning assay (Huang et al., 2015; Li et al., 2008; Liu et al., 2004; Liu et al., 2007; Liu et al., 2009; Qu et al., 2013a; Qu et al., 2013b). B–G. Venus YFP together with wild type human TUBB3 (B–C), R262C (D–E) or A302V (F–G) were electroporated into chick neural tubes and explants cultured with either netrin-1 cells (C, E and G) or control HEK293 cells (B, D and F). The scale bar is 100 µm. L. Quantification of axon turning. Data are mean ± s.e.m ***p<0.001 (One-way ANOVA with Tukey’s test for post-hoc comparisons). The axons from commissural neurons transfected with wild type TUBB3, but not R262C or A302V, were attracted to the netrin-1 source.