Figure 7.

PSI photoinhibition in pgrl1 cells exposed to phototrophic high light conditions can be rescued by the addition of DCMU. Cells acclimated to low-light (CL) conditions (TAP medium at 20 µmol photons m⁻² s⁻¹ illumination) were shifted to HL (200 µmol photons m⁻² s⁻¹)/photoautotrophic conditions for 24 h. A control culture was kept in low light/photoautotrophic conditions. For DCMU treatment (indicated as + DCMU), 10 µm DCMU was added upon shift to phototrophic conditions. Changes in protein abundance of the PSI subunit PSAD in pgrl1 and the wild type at CL and HL in the absence and presence of DCMU. Detection of the chloroplastic ATPase ATPB subunit was utilized as a loading control. Cells were adjusted to a total chlorophyll amount of 2.5 mg (100%) or 1.25 mg (50%) prior to protein separation by SDS-PAGE.