Figure 2.

Comparative analysis of intact N-glycopeptides from wild type and IM_Man1A. Applying IS-CID for the analysis of intact N-glycopeptides revealed a decreased number of MeHex and Pent in IM_Man1A in comparison to the wild type. Representative MS1 spectra for ITYATTAAAVTNANLSSYK are shown for wild type (A) and IM_Man1A (B), respectively. If not indicated otherwise, ions of charge state two are annotated. For all identified N-glycan compositions, the number of N-glycosites harboring this glycan is shown for the wild type (gray) and IM_Man1A (green; C). The N-glycan complexity is increasing from left (oligomannosidic, not methylated) to right (decorated, methylated) and N-glycan compositions have been grouped according to the presence of Pent and/or dHex (optional for sugars written in parenthesis). Only N-glycosites that have been identified in both strains were taken into account (n = 26). A comparison for all identified N-glycopeptides can be found in Supplemental Figure S2. Peptide sequences and their corresponding N-glycan compositions are listed in Supplemental Data S2.