Figure 6.
Comparison of N-glycan characteristics for wild type, IMMan1A, IMXylT1A, and IMMan1AxIMXylT1A. A, Comparison of N-glycan length, defined here as the sum of Hex and MeHex, (23 compared N-glycosites). B, Differences in N-glycan length (lengthIM − lengthWT) for N-glycosites identified in the wild type and respective IM strain. Some N-glycosites harboring multiple N-glycoforms could not be assigned to one of the categories (n.a.). C, Comparison of the percentage of MeHex in respect to N-glycan length (23 compared N-glycosites). D, Differences in the number of Pent (PentIM − PentWT) for N-glycosites that carried two Pent in the wild type. For each comparison, the legend indicates the total number of N-glycopeptides (A and C) and the total number of N-glycosites (B and D) for each strain, respectively. Results for progenies of genetic crossings with CC-124 have been included for the corresponding parental strain.