Figure 2.

Appearance and free Lys content of mature and developing seeds from different transgenic lines and their wild type (WT). A, Cross section of mature grains showing the aleurone layer and the mature endosperm. B, Free Lys content in mature seeds from the wild type, the two parental lines (GR-14 and 35S-15), and the pyramid HFL1 lines. HFL1-D and HFL1-N were mature seeds from the same HFL1 line with normal and dark-brown color appearance, respectively. C, Appearance of caryopsis at different developmental stages. D, Dynamic change of free Lys content during seed development. Error bars represent sd of three biological replicates, and different letters indicate statistical significance among transgenic plants and the wild type at P < 0.05. Bars = 3 mm.