Figure 11.

sHSP22 OX potentiates PIN1 trafficking and initiates more lateral roots. A, Internalization of proPIN1:PIN1-GFP in 5-d-old Col-0 and sHSP22 overexpressing Arabidopsis seedling roots upon adding a vesicle-trafficking inhibitor using 50 μm BFA for the indicated times. Inserted panels are a magnification of the denoted area. PI was used to stain the cell wall. Bar = 20 μm. B, sHSP22 overexpression and vector control seedlings germinated vertically for 5 d with similar length of roots were transferred to half-strength MS medium containing 0 and 50 nm NAA and 90 nm IAA and grown in a vertical position for additional 7 d. Bar = 0.5 cm. C, Statistic analysis of lateral root number of seedling shown in B. Error bars represent ± sd (n = 30), **P < 0.01 and *P < 0.05. D, Expression of GATA23 and SKP2B in vector control and sHSP22 OX seedlings. One-week-old vector control and sHSP22 OX seedlings were treated with 2 μm IAA for 0, 2, and 4 h, and the excised roots were harvested for RNA extraction and qRT-PCR. Transcript levels were normalized to ACTIN2 expression, and error bars represent ±sd of triplicates. **P < 0.01 and *P < 0.05.