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. 2017 Dec 29;176(3):2406–2425. doi: 10.1104/pp.17.01206

Figure 2.

Figure 2.

Expression of sHSP22 induced by IAA is dependent on ABI1. A, Microarray data for sHSP22 expression in 2-week-old wild-type and abi1-3 seedlings treated with 2 μm IAA for a time course of 0, 2, and 6 h. B, Confirmation of sHSP22 expression in A using qRT-PCR. C, Analysis of prosHSP22:sHSP22-GUS expression in the wild type and abi1-3. Five-day-old Col-0 and abi1-3 seedlings were treated with half-strength MS liquid supplemented with 0 or 5 μm IAA for 6 h and subjected to GUS staining. Bar = 2 mm. D, Expression of sHSP22 in response to 50 μm ABA treatment. Two-week-old Col-0 seedlings were treated with ABA for the time course of 0, 1, 2, 3, 4, and 5 h and harvested for RNA extraction and qRT-PCR. For B and D, sHSP22 transcript levels were normalized to ACTIN2 expression, and error bars represent ±sd of triplicates.