Table I. Segregation data from selfed progeny and a transmission efficiency analysis of the quadruple mutants.
Reciprocal crosses were performed between lrx heterozygous plants and wild-type (WT) plants. The transmission efficiency (TE) of gametes was calculated as follows: TE = number of progeny with the lrx allele/number of wild-type progeny × 100%. The observed TE was compared with the expected TE for normal gametes (100%). NA, Not applicable; TEF, female transmission efficiency; TEM, male transmission efficiency. For the χ2 test, degrees of freedom = 1, P < 0.05, χ2 > 3.84 or degrees of freedom = 2, P < 0.05, χ2 > 5.99.
| Parental Genotype (Female × Male) | Progeny Genotype |
TEF | TEM | χ2 | Expected |
|||
|---|---|---|---|---|---|---|---|---|
| +/+ | +/− | − /− | TE | χ2 | ||||
| lrx8(+/−) lrx9(−/−) lrx10(−/−) lrx11(−/−) ⊗ | 127 | 115 | 7 | NA | NA | 117 | NA | 5.99 |
| ♀WT × ♂ lrx8(+/−) lrx9(−/−) lrx10(−/−) lrx11(−/−) | 329 | 0 | NA | NA | 0% | 327 | 100% | 3.84 |
| ♀ lrx8(+/−) lrx9(−/−) lrx10(−/−) lrx11(−/−) × ♂WT | 231 | 172 | NA | 74.5% | NA | 8.34 | ||
| lrx8(−/−) lrx9(+/−) lrx10(−/−) lrx11(−/−) ⊗ | 120 | 128 | 32 | NA | NA | 57.4 | NA | 5.99 |
| ♀WT × ♂ lrx8(−/−) lrx9(+/−) lrx10(−/−) lrx11(−/−) | 227 | 38 | NA | NA | 13.7% | 179.8 | 100% | 3.84 |
| ♀ lrx8(−/−) lrx9(+/−) lrx10(−/−) lrx11(−/−) × ♂WT | 144 | 113 | NA | 78.5% | NA | 3.5 | ||
| lrx8(−/−) lrx9(−/−) lrx10(+/−) lrx11(−/−) ⊗ | 94 | 112 | 18 | NA | NA | 51.6 | NA | 5.99 |
| ♀WT × ♂ lrx8(−/−) lrx9(−/−) lrx10(+/−) lrx11(−/−) | 249 | 116 | NA | NA | 46.6% | 47.7 | 100% | 3.84 |
| ♀ lrx8(−/−) lrx9(−/−) lrx10(+/−) lrx11(−/−) × ♂WT | 170 | 173 | NA | 102% | NA | 0.012 | ||
| lrx8(−/−) lrx9(−/−) lrx10(−/−) lrx11(+/−) ⊗ | 161 | 223 | 44 | NA | NA | 64.72 | NA | 5.99 |
| ♀WT × ♂ lrx8(−/−) lrx9 (−/−) lrx10(−/−) lrx11 (+/−) | 224 | 77 | NA | NA | 34.4% | 70.8 | 100% | 3.84 |
| ♀ lrx8(−/−) lrx9(−/−) lrx10(−/−) lrx11(+/−) × ♂WT | 212 | 187 | NA | 88.2% | NA | 1.44 | ||