HIPP phylogeny. A, Diagrammatic representation of the phylogenetic relationship between A44 and five HIPP families (de Abreu-Neto et al., 2013). A44 is related to Arabidopsis HIPP subfamily II and most closely related to HIPP26 (for full phylogeny, see Supplemental Fig. S1). B, Multiple alignments of HIPP26 proteins from Arabidopsis (Arath), N. benthamiana (Benth), tomato, and potato with A44. The alignments were made using MUSCLE with default parameters (Edgar, 2004). The line below the alignment marks conserved positions: *, single, fully conserved residue; :, strong group, fully conserved, scoring > 0.5 in the Gonnet PAM 250 matrix; ., weaker group, fully conserved, scoring ≤ 0.5 in the Gonnet PAM 250 matrix. C, Percentage sequence identities for the four HIPP26 proteins and A44 shown in the alignment in B.