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. 2017 Dec 19;176(3):2365–2375. doi: 10.1104/pp.17.01305

Figure 5.

Figure 5.

Disruption of the second B-box in BBX21 impairs its transcriptional activity. A, Schematic representation of various constructs used in the transient transfection assay in protoplasts. Arrow after the 35S promoter indicates the transcriptional start site. D-n-A indicates the Ala substitution of the three Asp residues in the B-boxes at positions 20, 75, and 84 (n); −450 indicates the length of the HY5 promoter carrying a wild-type or a mutated T/G-box that was fused to the firefly luciferase to create the reporter constructs. B, Bar graph showing activation by wild-type, various mutated versions, and two respective B-box domains of BBX21 on the ProHY5:LUC reporter and the effect of mutating the T/G-box (CACGTT was mutated to ACCGGG) in the HY5 promoter. Error bars represent se (n = 3).