Disruption of the second B-box in BBX21 impairs its ability for activating the HY5 expression in Arabidopsis. Quantitative RT-PCR analyses of HY5, CHS, CHI, DFR, LDOX, F3H, and F3′H transcript levels in Col, bbx21-1, BBX21, and various mutated BBX21 transgenic seedlings grown under W light for 4 d.