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Somatic cell chromosomes of plant F1-15 labeled with a telomeric DNA probe (green) and an EPSPS probe (red). Note the size variation in the sECCAEs and lack of telomeric signals (insets in A and B). In addition to the expected pericentromeric EPSPS signals on two chromosomes (white arrows), de novo formed EPSPS-FISH foci (arrowheads) were observed on an additional one to four chromosomes in 90% of the cells (A–D). Green arrows point to the ECCAE. Bars = 5 µm.
