Figure 7.

ERF17 is essential for Chl degradation in apple fruit. A, Apple fruit peel coloration. Three ERF17 genotypes were overexpressed in apple fruits by two A. tumefaciens-mediated transient transformation methods (injection and infiltration). Apples injected and infiltrated with the empty pBI1300 vector were used as controls. B, Chl content around the infiltration sites of apple peels in mg g⁻¹ fresh weight (FW). C, Relative expression levels of MdERF17, MdNYC, and MdPPH in apple fruit peels around the infiltration sites determined using reverse transcription real-time quantitative PCR (RT-qPCR). An actin fragment was amplified as an internal control. Values are means ± sd (n = 3) in B and C. Asterisks indicate statistically significant differences by Student’s t test (**, P < 0.01) compared with the control. ND, Not detected.