Figure 3.

CKX1 homodimerizes in a BiFC assay. A, Confocal microscopy analysis of BiFC in N. benthamiana epidermal leaf cells reveals interaction between NVen-CKX1 and CVen-CKX1, as apparent from the reconstitution of the Venus-derived fluorescence (yellow). The microscopy was performed 2 d after infiltration (DAI). B, The NVen-CKX1/CVen parent vector shows no background BiFC signal 2 DAI. Successful transformation is evident from the cyan mTq2 signal in the Golgi. No BiFC signal was observed even after prolonged incubation (3 DAI). Images in A and B were captured using identical confocal settings. C, NVen-CKX1/CVen-CKX1 BiFC fluorescence signal localizes predominantly to the ER. Yellow, Venus BiFC; magenta, RFP-p24. Bars = 50 μm (A and B) and 5 μm (C).