Figure 5.
The CKX1 N terminus directs the homooligomerization and targeting to the ER. A, Co-IP assay for the detection of homooligomerization mediated by the CKX11-79 N-terminal fragment. The myc-CKX1 protein was coexpressed transiently with CKX11-79-GFP or CKX1-GFP in N. benthamiana, and the protein extracts were used for immunoprecipitations (IP) with anti-GFP antibody followed by immunoblot detection with anti-myc antibody. The left gel shows the input (10 µg of the crude extract used for Co-IP assay); the right gel shows the pellet fractions from the Co-IP assays. The input control for CKX11-79-GFP and CKX1-GFP is shown in Supplemental Figure S4. B, Confocal microscopy analysis of N. benthamiana leaf epidermal cells coexpressing CKX11-79-GFP (green) with the ER marker RFP-p24 (magenta). The microscopy was performed 2 DAI. Bars = 5 μm.