Figure 1.

Shoot regeneration is inhibited in the dcc1 mutant. A to C, Callus morphology (A), frequencies of shoot regeneration from calli (B), and number of shoots per callus (C) in wild-type Col-0, dcc1, and dcc1 ProDCC1:DCC1 cultured on SIM at the indicated times. D, Expression pattern of DCC1 detected by in situ hybridization in calli of Col-0 at the indicated times on SIM. E, Expression pattern of DCC1 detected by GUS staining in calli of ProDCC1:DCC1-GUS transgenetic lines at the indicated times on SIM. S0 represents calli cultured on callus induction medium (CIM) at 6 d using roots as explants before transfer to SIM. S8, S12, S16, S20, S24, and S28 indicate calli cultured on SIM at 8, 12, 16, 20, 24, and 28 d, respectively. se values were calculated from three sets of biological replicates, and more than 100 calli were examined in each replicate. Asterisks indicate significant differences: **, P < 0.01 (Student’s one-tailed t test). Bars = 500 µm.