Figure 12.

ROS levels are associated with the shoot regeneration capacities of different ecotypes in Arabidopsis. A, DAB staining showing ROS levels in calli of different ecotypes of Hap1 cultured on SIM at 16 d. B, DAB staining showing ROS levels in calli of different ecotypes of Hap2 cultured on SIM at 16 d. C, Shoot regeneration frequencies from calli of different ecotypes cultured on SIM at 28 d. D, Number of shoots per callus of different ecotypes cultured on SIM at 28 d. E, Relative staining intensity of ROS in calli of different ecotypes cultured on SIM at 16 d. se values were calculated from three sets of biological replicates, and more than 100 calli were examined in each replicate. Asterisks indicate significant differences: **, P < 0.01 (Student’s one-tailed t test). Bars = 500 µm.