Transcript levels of master genes involved in shoot regeneration. Transcript levels of master genes for callus formation (A) and shoot meristem induction (B) during shoot regeneration are shown. C3 and C6 represent calli cultured on CIM at 3 and 6 d, respectively, using roots as explants before transfer to SIM. S4, S8, S12, and S16 indicate calli cultured on SIM at 4, 8, 12, and 16 d, respectively. se values were calculated from three sets of biological replicates. Asterisks indicate significant differences: **, P < 0.01 (Student’s one-tailed t test).